Western blot analysis of NANS in extracts of various cell lines. Samples were incubated with NANS Polyclonal antibody (Product # PA5-96300) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Western Blot analysis of NANS in extracts of various cell lines using NANS Polyclonal Antibody (Product # PA5-96300) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supplier Page from Thermo Fisher Scientific for NANS Antibody